AMPLIFICATION OF STAINING BY ALCIAN BLUE AND SIMILAR INGRAIN DYES
نویسندگان
چکیده
منابع مشابه
Temperature-dependent staining reaction of chromatin by Alcian blue.
After treatment with Alcian blue at high temperatures, nuclei from chicken blood smears showed an intense staining reaction, which proved to be dependent on the DNA content of chromatin. The possibility that interactions between the phthalocyanine chromophore and unpaired bases accounts for this temperature-dependent staining of chromatin is briefly discussed.
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Since Steedman ( 1950) has introduced it into histochemistry, alcian blue has been advocated to reveal a staining selectivity which depends upon the presence of acidic groups in mucopolysaccharides (Mowry, 1956; Pearse, 1960). As a plausible ground of this concept the fact has been presented that the alcian blue staining of polysaccharides can be increased in intensity, when acidic groups are i...
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Difficulties in obtaining reproducible and accurate enumeration of circulating basophils with existing techniques have hampered investigation of this infrequent cell population. A new basophil staining method is described that employs alcian blue dye for staining of heparin within basophils at low pH and in the presence of lanthanum ions. Basophil recognition is facilitated by reducing nonspeci...
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In the atrioventricular canal (AVC) and outflow tract (OT) of the developing heart, endothelial cells transform specifically to mesenchymal cells. The mesenchymal cells migrate into the underlying acellular matrix termed cardiac jelly and form endocardial cushion tissue. It is believed the that the highly hydrated nature of cardiac jelly is ascribed to sulfated glycoconjugates in the components...
متن کاملModified double skeletal staining protocols with Alizarin red and Alcian blue in laboratory animals
Abstract Purpose: Skeletal staining is a way to study the effect of many chemical or herbal substances on development of bones and cartilages in order to record the level of probable deformity.This article aimed to present a modified protocol to make all skeletal studies on laboratory animals possible. Materials and Methods: The important notes about skeletal staining were fixing by ethan...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1972
ISSN: 0022-1554,1551-5044
DOI: 10.1177/20.9.750